Bioengineering basics, biometrology, and biocivics with ganoderma lucidum
Abstract/Contents
- Abstract
- Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on unicellular organisms or filamentous fungi in liquid bioreactors and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on aerobic solid-state fermentation (SSF) and wood-degrading fungi could enable more direct and sustainable means of manufacture, novel products, market pull for the biomaterials industry, reduced time to market, and reduced production costs. However, basic methods for cultivating wood-degrading fungi and the production of mycological materials are often ad hoc and not readily reproducible. Additionally, existing genetic engineering tools for wood-degrading fungi are not standardized or readily available. Furthermore, the global need for practitioners skilled in fungal biotechnology is outgrowing the available workforce at a time when civic engagement with the biological sciences by the general public is being poorly supported. To address the aforementioned needs, I have developed standard materials, measures, methods, and educational programs enabling bioengineering basics, biometrology, and biocivics with Ganoderma lucidum. Specifically, I developed standard materials, measures, and methods enabling simple laboratory culture of wood-degrading fungi. I established Ganoderma lucidum 10597-SS1 as a reference strain, PringlesTM as a regularized widely-available reference substrate, a simple plate-based assay for standardized radial extension measurements, and a relative extension unit framework for reducing extrinsic effects on measurements of growth that supports sharing of measurement data across locations. So established, I coordinated an interlaboratory study to enable community-based metrology for wood-degrading fungi in simple laboratory conditions and published a peer-reviewed paper describing our work. I co-authored a peer-reviewed White Paper to identify opportunities for reuse of materials, measures, and methods supporting reproducible science and reliable production with fungal biotechnology. Finally, I co-developed a culturally relevant education program, BioJam, to engage youth from underserved communities and nurture equity in biotechnology literacy and governance; and I also co-founded a public benefit organization, Xinampa, whose mission is to cultivate an equitable bioeconomy on the Central Coast of California and form nucleation points for practitioners from the community to develop public-interest biotechnologies that support their community. Taken together, my contributions should begin to advance distributed mycological production in support of all earthlings and the planet.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Perez, Rolando Cruz |
|---|---|
| Degree supervisor | Endy, Andrew D |
| Thesis advisor | Endy, Andrew D |
| Thesis advisor | Brown, Bryan Anthony |
| Thesis advisor | Peay, Kabir |
| Thesis advisor | Salit, Marc L |
| Degree committee member | Brown, Bryan Anthony |
| Degree committee member | Peay, Kabir |
| Degree committee member | Salit, Marc L |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Rolando Cruz Perez. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2020. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Rolando Cruz Perez
- License
- This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).
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