Mixotrophy and polyhydroxyalkanoates in type II methanotrophic bacteria
Abstract/Contents
- Abstract
- There is worldwide concern about pollution caused by non-biodegradable petroleum-based plastics and carbon emissions in the atmosphere. Specifically, the natural abundance of cheap methane-rich mixtures such as biogas (CH4 and CO2) and natural gas (primarily CH4 and C2H6) has increased atmospheric greenhouse gas (GHG) emissions. Type II methanotrophs are organisms that can potentially address both of these problems by metabolizing these methane-rich mixtures to sequester the carbon into produce high-value biodegradable plastics in the polyhydroxyalkanoate (PHA) family such as polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-hydroxyvalerate (PHBV). Through this dissertation, I demonstrate that Type II methanotrophs can sequester CO2 when using CH4 as the sole electron donor during cell growth and PHB accumulation, and I also provide evidence of CO2 incorporation into the PHB and PHBV biopolymer. To establish the effects of natural gas, I show PHB production from ethane alone in Type II methanotroph, Methylocystis parvus OBBP. The dissertation culminations with an evaluation of the impacts of CH4 and C2H6 on Methylocystis parvus OBBP cell growth and PHB accumulation. Overall, the findings in this dissertation contribute to our understanding about the metabolic versatility of Type II methanotrophs which can be harnessed in order to address significant global problems related to climate change, and plastic pollution.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Galega, Wakuna Mojoko |
|---|---|
| Degree supervisor | Criddle, Craig |
| Thesis advisor | Criddle, Craig |
| Thesis advisor | Waymouth, Robert M |
| Thesis advisor | Welander, Paula |
| Degree committee member | Waymouth, Robert M |
| Degree committee member | Welander, Paula |
| Associated with | Stanford University, Civil & Environmental Engineering Department. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Wakuna Mojoko Galega. |
|---|---|
| Note | Submitted to the Civil & Environmental Engineering Department. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Wakuna Mojoko Galega
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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