Characterization of zeta-tubulin and discovery of the ZED tubulin module
Abstract/Contents
- Abstract
- Microtubules and microtubule-based structures play key roles in a variety of cellular processes, including cellular trafficking, motility, division, and signaling. In most animal cells, microtubules are organized by centrosomes, which contain a pair of specialized microtubule-based structures called centrioles surrounded by a meshwork of proteins known as pericentriolar material. In these cells, one centriole can elongate a single primary cilium that performs a number of sensory and signaling functions. In many specialized cell types of the adult vertebrate body plan, however, cells contain tens to hundreds of centrioles that nucleate motile cilia. Defects in centrioles, primary cilia, and motile cilia cause human diseases, both congenital and acquired. In this thesis, we have undertaken a study of zeta-tubulin, a member of the tubulin superfamily of proteins that are essential to the structure and function of microtubules and microtubule-based structures. Zeta-tubulin is a multiciliated-cell specific protein that orients the centrioles via localization to an important appendage known as the basal foot. As a confirmation of the co-conservation of zeta-, epsilon-, and delta- tubulin, we show that zeta- and epsilon-tubulin also co-localize at basal feet in multiciliated cells, suggesting concerted function of these ZED tubulins. We provide the basis for future studies of ZED tubulin biochemical function by purification of these tubulins from insect cells using the baculovirus expression system. Finally, we investigated the transcriptome of mouse multiciliated cells using bioinformatic approaches and utilized Xenopus datasets to discover potential new multiciliated cell specific proteins.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Turk, Erin Nicole |
|---|---|
| Associated with | Stanford University, Cancer Biology Program. |
| Primary advisor | Stearns, Tim |
| Thesis advisor | Stearns, Tim |
| Thesis advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Goodman, Miriam Beth |
| Thesis advisor | Straight, Aaron, 1966- |
| Advisor | Cyert, Martha S, 1958- |
| Advisor | Goodman, Miriam Beth |
| Advisor | Straight, Aaron, 1966- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Erin Nicole Turk. |
|---|---|
| Note | Submitted to the Program in Cancer Biology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Erin Nicole Turk
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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