Fibroblasts Show Attenuated Wound Healing Phenotype in Dermal Melanoma in Mice

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Abstract/Contents

Abstract: Fibroblasts play a critical role in the secretion of the extracellular matrix. As such, they are highly involved in the response to wounding and the wound healing process. Tumors recruit fibroblasts to establish a tumorigenic niche and aid cancer progression, a process in which fibroblasts undergo a phenotypic transition and differentiate into cancer-associated fibroblasts (CAFs). We examined this phenotype in the setting of dermal melanoma cells in mice (BrafCA, PTENLoxP, Tyr:CreERT2). Mice with endogenous and grafted dermal melanoma were wounded on the site of the tumor and their wound healing ability was then evaluated based on the morphology of the connective tissue using a novel automated connective tissue analysis methodology. Our findings indicate that CAFs are unable to recapitulate normal fibroblast wound healing behavior. The histology of the healed tumors instead closely resembled normal tumor biology, indicating that once recruited and activated by a tumor CAFs terminally differentiate into a distinct phenotype. CAFs may thus offer a cellular target of therapeutic significance in skin oncology.

Type of resource	text
Date created	June 2021

Author	da Silva, Oscar
Primary advisor	Longaker, Michael
Advisor	Gozani, Or
Degree granting institution	Stanford University, Department of Biology, 2021

Subject	Biology
Subject	Cancer Biology
Subject	Fibroblast
Subject	Melanoma
Subject	Connective Tissue
Subject	Stanford Department of Surgery
Genre	Thesis

Location	https://purl.stanford.edu/qy623xp2649

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License: This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).

Preferred Citation: da Silva, Oscar and Longaker, Michael and Gozani, Or. (2021). Fibroblasts Show Attenuated Wound Healing Phenotype in Dermal Melanoma in Mice. Stanford Digital Repository. Available at: https://purl.stanford.edu/qy623xp2649

Undergraduate Theses, Department of Biology, 2020-2021

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