Soft matter approaches towards understanding cell type diversity
Abstract/Contents
- Abstract
- From purely physical models to sequencing technology, soft matter science provides an expansive inventory of tools for gaining a deeper understanding of diverse cell types that assemble into tissues, organs, and organisms. My thesis uses soft matter approaches, both conceptual and technical, to analyze cell type diversity. First, I take a physics approach to characterize the organizational pattern of the brain structure in a basal organism, to show that cell type-specific interactions govern cellular packing in space. These findings extend the classical notion of particulate packing by including cell type diversity as a previously neglected orthogonal dimension in order to understand the packing phenomena in biological matter. Second, I developed a double emulsion droplet platform to encapsulate and phenotype single cells with high throughput and wide applicability. To use these emulsion droplets as picoreactors for measuring gene expression, I characterize and overcome several key challenges in performing small-volume reactions to significantly improve picoscale droplet-based PCR assays. This demonstration opens up a new path towards enriching for rare cell types through droplet-based gene expression screening in order to access otherwise concealed cell type-specific information.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2021; 2021 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Khariton, Margarita |
|---|---|
| Degree supervisor | Wang, Bo, (Researcher in Bioengineering) |
| Thesis advisor | Wang, Bo, (Researcher in Bioengineering) |
| Thesis advisor | Bintu, Lacramioara |
| Thesis advisor | Fordyce, Polly |
| Degree committee member | Bintu, Lacramioara |
| Degree committee member | Fordyce, Polly |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Margarita Khariton. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/qx569ds1499 |
Access conditions
- Copyright
- © 2021 by Margarita Khariton
- License
- This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).
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