Restructuring the 3D chromosome for gene regulation
Abstract/Contents
- Abstract
- Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. This has prevented a comprehensive understanding of chromatin loop formation and maintenance. To address this, a novel method for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9) was engineered to selectively and reversibly establish chromatin loops. The power of this technology to selectively modulate gene expression at targeted loci is demonstrated. Further, use of CLOuD9 enabled identification of the RNA helicase DDX5 as a novel chromatin structure regulation factor responsible for stabilization of de novo chromatin loops. This has particularly important implications in cancer, where destabilized chromatin structure is frequently observed. Collectively, findings from this body of work will be critical to obtaining a more comprehensive understanding of how chromatin dynamics specifically impact transcription apparatus to facilitate oncogenesis, and may further shed light on useful ways to design novel therapies for more targeted cancer treatments.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Morgan, Stefanie |
|---|---|
| Degree supervisor | Wang, Kevin K. W |
| Thesis advisor | Wang, Kevin K. W |
| Thesis advisor | Bassik, Michael |
| Thesis advisor | Mallick, Parag, 1976- |
| Thesis advisor | Oro, Anthony, 1958- |
| Degree committee member | Bassik, Michael |
| Degree committee member | Mallick, Parag, 1976- |
| Degree committee member | Oro, Anthony, 1958- |
| Associated with | Stanford University, Cancer Biology Program. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Stefanie Morgan. |
|---|---|
| Note | Submitted to the Cancer Biology Program. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Stefanie Lynn Morgan
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
Also listed in
Loading usage metrics...