Ms8 microarray data 2007 - DongXue Wang

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Abstract/Contents

Abstract: Maize anther ontogeny is complex with expression of more than 30,000 genes over four days of cell proliferation, cell fate acquisition, and the start of meiosis. Although many male-sterile mutants disrupt these key steps, few have been investigated in detail. The terminal phenotypes of maize male-sterile 8 (ms8) are small anthers exhibiting meiotic failure. Here we document much earlier defects: ms8 epidermal cells are normal in number but fail to elongate, and there are fewer, larger tapetal cells that retain rather than secrete their contents. ms8 meiocytes separate early, have extra space between them occupied by excess callose, and the meiotic dyads abort. Thousands of transcriptome changes occur in ms8 including ectopic activation of genes not expressed in fertile siblings, failure to express some genes, differential expression compared to fertile siblings and about 40% of the differentially expressed transcripts appear precociously. There is a high correlation between mRNA accumulation assessed by microarray hybridization and qRT-PCR. Sixty-three differentially expressed proteins were identified after 2-D gel electrophoresis followed by LC/MS/MS (liquid chromatography tandem mass spectroscopy), including those involved in metabolism, plasmodesmatal remodeling, and cell division. The majority of these were not identified by differential RNA expression demonstrating the importance of proteomics to define developmental mutants.

Type of resource	software, multimedia
Date created	[ca. 2007]

Author	Wang, Dongxue

Location	https://purl.stanford.edu/qt699xn3153

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License: This work is licensed under an Open Data Commons Public Domain Dedication & License 1.0.

Preferred Citation: Wang, D.-X. J. A. Oses-Prieto, K. H. Li, J. F. Fernandes, A. L. Burlingame, and V. Walbot.
2010. The male sterile 8 mutation of maize disrupts the temporal progression of the
transcriptome and results in mis-regulation of metabolic functions. Plant J. 63: 939-951. doi: 10.1111/j.1365-313X.2010.04294.x

Research data from Walbot Lab at Stanford University

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