Community dynamics and variation of the murine intestinal microbiota in health and disease
Abstract/Contents
- Abstract
- The importance of the intestinal microbiota to the health of an organism is well recognized. Alterations in the composition of the microbiota are associated with a number of diseases. However, the extent to which the microbiota varies across healthy individuals and time and the causes of this variation are still not fully understood. In order to distinguish between microbial community states of health and disease, the range of healthy microbial communities must be determined. In addition, the effect of perturbations on the microbiota is an area of active research. Recent studies indicate that intestinal infections can have a significant impact on the composition of intestinal microbiota. However, the extent and duration of these changes during different infections have not been fully characterized. My aims were to characterize the range of healthy microbiota in an inbred mouse population over time and to determine the dynamics of alterations in the microbiota during disease. I also wanted to determine if certain characteristics of the microbiota make animals more or less susceptible to infection. In this work, 46 individual healthy mice were sampled over time to assess the range of microbial communities in health. A mouse model of Salmonella enterica serovar Typhimurium infection was used to assess the impact of infection on the microbiota. Mice were sampled prior to infection and over the course of infection. This work finds that the microbiota of healthy mice varies significantly over time and among individuals. However, there are stable differences among individual mice, which may be established at the point of weaning when adult microbial communities develop. In addition, this work finds significant changes in the microbiota during infection that differ with the level of infection. This data also shows that there are some taxa whose abundance prior to infection correlates with outcome of infection. This work provides insights into the dynamics of the microbiota in health and disease and its impact on the health of the host.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2011 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Hoy, Emmy Evangeline |
|---|---|
| Associated with | Stanford University, Department of Microbiology and Immunology |
| Primary advisor | Relman, David A |
| Primary advisor | Theriot, Julie |
| Thesis advisor | Relman, David A |
| Thesis advisor | Theriot, Julie |
| Thesis advisor | Monack, Denise M |
| Thesis advisor | Schneider, David (David Samuel) |
| Thesis advisor | Sonnenburg, Justin, 1973- |
| Thesis advisor | Spormann, Alfred M |
| Advisor | Monack, Denise M |
| Advisor | Schneider, David (David Samuel) |
| Advisor | Sonnenburg, Justin, 1973- |
| Advisor | Spormann, Alfred M |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Yana Emmy Evangeline Hoy. |
|---|---|
| Note | Submitted to the Department of Microbiology and Immunology. |
| Thesis | Ph. D. Stanford University 2011 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2011 by Emmy Evangeline Hoy
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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