Mechanics at the cadherin-keratin interface
Abstract/Contents
- Abstract
- Mechanical forces are ubiquitous in living tissues. From embryonic development to adult tissue repair, cells coordinate forces and mechanical properties to control tissue shape. In addition to a remarkable ability to remodel and grow, tissues must also be mechanically resilient to strains associated with motion and external force. In this thesis two classes of tools are employed to better understand shape and tension homeostasis in living cells. The first project describes the development and use of an artificial extracellular matrix for evaluating the response of pluripotent cell colonies to mechanical cues. This system reveals an interplay between colony morphology and substrate mechanics in pluripotent cells, a potentially relevant finding for models of the epiblast from which the cells are derived. The second project describes the development and use of a tension reporter for desmoplakin, a linker protein that connects the intermediate filament cytoskeleton to the cell-cell junction. Measurements of this sensor in epithelial monolayers revealed that the this linkage responds to acute deformations, but does not bear significant loads during homeostasis in culture. These measurements provide an essential step toward understanding the molecular origins of the mechanics of living tissues.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2018; ©2018 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Price, Andrew John |
|---|---|
| Degree supervisor | Dunn, Alexander Robert |
| Thesis advisor | Dunn, Alexander Robert |
| Thesis advisor | Pruitt, Beth |
| Thesis advisor | Weis, William I |
| Degree committee member | Pruitt, Beth |
| Degree committee member | Weis, William I |
| Associated with | Stanford University, Department of Biophysics. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Andrew John Price. |
|---|---|
| Note | Submitted to the Department of Biophysics. |
| Thesis | Thesis Ph.D. Stanford University 2018. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2018 by Andrew John Price
- License
- This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).
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