Designing bioinks for 3D bioprinting in gel-based support baths
Abstract/Contents
- Abstract
- 3D bioprinting has emerged as a powerful tool to pattern extracellular matrix (ECM) and living cells to produce biological structures that mimic native tissues and organs ex vivo. Bioinks, the cell- and ECM-laden mixtures used during bioprinting, have primarily been composed of biopolymers appropriated from traditional 3D tissue engineering to form hydrogels following printing. These materials are not particularly well suited to meet the challenges introduced through bioprinting, namely, cell sedimentation, mechanical damage while printing, printing in gel-based support baths, and tunability of the mechanics and bioactivity of the biopolymer hydrogels. New bioink materials can be produced through intentional design of the crosslinking scheme of the hydrogel network before and after bioprinting into a gel-based support bath. The work presented in this thesis explores the benefits of designing new bioink materials for 3D bioprinting that move beyond the simplest crosslinking strategies used in traditional tissue engineering materials. Specifically, bioinks that employ two different stages of crosslinking, a first-stage crosslinking before the material is printed and a second stage following printing in a bath, prove to be effective for 3D bioprinting. One example of the increased versatility of bioinks from a dual-crosslinking approach is a bioprinted stem cell expansion lattice for stem cell manufacturing made using a traditional bioink material, sodium alginate. The second half of this thesis introduces two new crosslinking schemes that are made possible only by bioprinting into a gel-based support bath. Both bioorthogonal and hydrazone dynamic covalent chemistries are explored for producing new bioinks. The work put forward in this thesis is a step toward designing new bioinks for 3D bioprinting. An array of crosslinking schemes for bioinks are presented alongside creative uses of existing biopolymers to produce more adaptable bioinks
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Lindsay, Christopher Dean |
|---|---|
| Degree supervisor | Heilshorn, Sarah |
| Thesis advisor | Heilshorn, Sarah |
| Thesis advisor | Appel, Eric (Eric Andrew) |
| Thesis advisor | Chaudhuri, Ovijit |
| Degree committee member | Appel, Eric (Eric Andrew) |
| Degree committee member | Chaudhuri, Ovijit |
| Associated with | Stanford University, Department of Materials Science and Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Christopher D. Lindsay |
|---|---|
| Note | Submitted to the Department of Materials Science and Engineering |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Christopher Dean Lindsay
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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