Microfluidic devices for studies of single cell wound repair, regeneration, and other biological phenomena
Abstract/Contents
- Abstract
- Microfluidics has enabled a wide range of biological studies. The objective of my thesis dissertation is to apply microfluidics for studies of wound repair and regeneration, as well as to use droplet microfluidics to study stochastic biological phenomena. My thesis covers three parts: In Chapters 2 and 3, I develop the microfluidic guillotine for high-throughput studies of wound repair in Stentor coeruleus. We were able to bisect over 150 cells in 3 minutes, which is 200 times faster than current methods, while achieving approximately 95% cell survival. I also develop quantitative and qualitative assays to assess wound repair. With these tools, we measured the wound repair time in Stentor and discovered the occurrence of three mechanical modes of wound repair, as well as Ca2+ activated wound repair processes. In Chapters 4 and 5, I further develop the microfluidic guillotine to bisect multicellular structures and show that organoids bisected by the guillotine can also regenerate. Furthermore, I optimize cutting performance by developing a method using the Nanoscribe, a sub-micron resolution 3D printer, to fabricate the blade at an angle. My work on the microfluidic guillotine in Chapters 2 -- 5 has been patented and we are in the process of commercializing the technology. Finally, in Chapters 6 and 7, I used the uniquely high-throughput, low volume nature of droplet microfluidics to study stochastic biological phenomena. I developed theory to understand the impact of drop-to-drop variance in studies of single cell phenotypic heterogeneity. Using droplet microfluidics also lead to the discovery of hundreds of novel RNAs that can be replicated by T7 RNAP, whereas before only five were known. This lead to my collaborator discovering the origin and replication mechanism of these replicating RNAs. We have submitted a provisional patent on this technology
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Blauch, Luke Richard |
|---|---|
| Degree supervisor | Tang, Sindy (Sindy K.Y.) |
| Thesis advisor | Tang, Sindy (Sindy K.Y.) |
| Thesis advisor | Chaudhuri, Ovijit |
| Thesis advisor | Marshall, Wallace F, 1968- |
| Degree committee member | Chaudhuri, Ovijit |
| Degree committee member | Marshall, Wallace F, 1968- |
| Associated with | Stanford University, Department of Mechanical Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Luke R. Blauch |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Luke Richard Blauch
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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