Pluripotent stem cell morphogenesis in engineered hydrogels as a model of early human development
Abstract/Contents
- Abstract
- Human embryogenesis involves a series of complex morphogenetic events, wherein human pluripotent stem cells (hPSCs) give rise to all the tissues in the human body. Mechanisms of embryogenesis cannot be studied directly in human embryos due to ethical concerns and technological limitations. Yet, these mechanisms hold the key for taking stem-cell-based therapies to the clinic. To tackle this, there has been a tremendous effort recently toward developing hPSC-based models of the embryo, but current studies lack mechanistic insights as well as reproducibility, due to use of extracellular matrices such as Matrigel which are poorly defined and heterogeneous with limited tunability of matrix properties. In this dissertation, I will first demonstrate the use of engineered alginate hydrogels for studying the impact of hydrogel mechanical properties on hPSC behavior, with hPSCs forming human epiblast-like structures in specific hydrogels. The human epiblast forms a central fluid-filled lumen during embryogenesis which is crucial for proper establishment of the body axes. Using the hPSC-based model of human epiblast, I next unearth the mechanism of epiblast lumen formation. Overall, this body of work demonstrates the power of embryo models and engineered hydrogels, and greatly advances our understanding of the self-organization mechanisms that drive human embryo development.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Indana, Dhiraj |
|---|---|
| Degree supervisor | Chaudhuri, Ovijit |
| Thesis advisor | Chaudhuri, Ovijit |
| Thesis advisor | Bhutani, Nidhi |
| Thesis advisor | Levenston, Marc Elliot |
| Degree committee member | Bhutani, Nidhi |
| Degree committee member | Levenston, Marc Elliot |
| Associated with | Stanford University, School of Engineering |
| Associated with | Stanford University, Department of Mechanical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Dhiraj Indana. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/pf792dk2298 |
Access conditions
- Copyright
- © 2023 by Dhiraj Indana
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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