Formation and separation mechanics of droplet interface bilayers
Abstract/Contents
- Abstract
- Phospholipid bilayers are the major component of cell membranes that regulate ion permeation and molecular transport. Droplet interface bilayers (DIBs), artificial phospholipid bilayers formed between two lipid-coated aqueous droplets in non-polar phase, provide stable and reproducible bilayers that can be conveniently imaged using brightfield camera and fluorescence tools. In this dissertation, we created an in house developed platform to generate the DIBs formed between a pendant and a sessile droplet and then separated the formed bilayers using step strain displacements. We track the contact angle and the bilayer radius at the bilayer interface to study the bilayer separation mechanics, and we implement a mathematical model to simulate the evolution of the angles and radius mentioned above. Then we focused on the formation and separation mechanics of the DIBs under the salt type and concentration effects. In addition, we built a platform compatible with confocal microscopes to generate and apply controlled deformations on artificial bilayer while simultaneously probing membrane stresses through fluorescence lifetime imaging. We incorporated a fluorescent lipid tension reporter (FliptR), a synthesized molecule that can monitor the changes in membrane tension, to study the formation and separation dynamics of a droplet interface bilayer.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Huang, Yaoqi |
|---|---|
| Degree supervisor | Fuller, Gerald G |
| Thesis advisor | Fuller, Gerald G |
| Thesis advisor | Dunn, Alexander Robert |
| Thesis advisor | Mai, Danielle |
| Degree committee member | Dunn, Alexander Robert |
| Degree committee member | Mai, Danielle |
| Associated with | Stanford University, Department of Chemical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Yaoqi Huang. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/pb966xs8752 |
Access conditions
- Copyright
- © 2022 by Yaoqi Huang
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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