Dual-stage crosslinking hydrogels for cell extrusion in bioprinting and transplantation applications
Abstract/Contents
- Abstract
- Nature has provided the human body with complex systems of wound healing that enable us to overcome many of the assaults inflicted upon us. Typically, these systems are categorized as either regeneration, where the native tissue and its structure are replaced, or repair, where a different tissue structure forms in the wound site. While there are several strategies in the literature to achieve this goal, this thesis focuses on two: the delivery of regenerative aids in vivo to promote regeneration at the wound site and the development of tissue replacements ex vivo for future implantation. This thesis proposes two families of hydrogels that each employ two stages of crosslinking to specifically tune material properties for various biomedical applications. The use of protein engineered materials to create a shear-thinning, injectable hydrogel is central to both. For promotion of in vivo regenerative wound healing, cell transplantation within a biomaterial scaffold will be explored. This thesis explores the use of Shear-thinning Hydrogel for Injectable Encapsulation and Long-term Delivery, or SHIELD, for Schwann cell delivery in vivo for a spinal cord injury model. This material uses a thermal secondary crosslinking mechanism for altered material properties at body temperature. To be able to engineer tissue replacements ex vivo, bioprinting has emerged as a promising technique due to its ability to precisely pattern multiple cell types into structures found in native tissues. This work discusses the design and development of a dual-stage crosslinking bio-ink for use in 3D printing, named Recombinant-protein Alginate Platform for Injectable Dual-crosslinked ink or RAPID ink, to overcome some of these challenges. This ink uses an ionic secondary crosslinking mechanism to maintain the printed structure and allow for full cell hydration during printing.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2017 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Dubbin, Karen Ruth |
|---|---|
| Associated with | Stanford University, Department of Materials Science and Engineering. |
| Primary advisor | Heilshorn, Sarah |
| Thesis advisor | Heilshorn, Sarah |
| Thesis advisor | Appel, Eric (Eric Andrew) |
| Thesis advisor | Plant, Giles |
| Advisor | Appel, Eric (Eric Andrew) |
| Advisor | Plant, Giles |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Karen Ruth Dubbin. |
|---|---|
| Note | Submitted to the Department of Materials Science and Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2017. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2017 by Karen Ruth Dubbin
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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