Characterizing how coronary arteries expand after embryonic development: the Postnatal Wave of Arterial Remodeling
Abstract/Contents
- Abstract
- Coronary artery disease (CAD) is the deadliest disease in the world. Thus, understanding the mechanisms behind coronary artery development could lead to new therapeutics to treat CAD. Although advances in cardiovascular research have broadened our understanding of cardiovascular development, most are focused on early embryonic stages. This project aims to investigate a less understood coronary arterial developmental event: postnatal coronary artery remodeling. A newly developed imaging technique called iDISCO allows for optical clearing of tissue and visualization of their integrated structures, such as neurons in brain tissue. By applying this technique to wild-type mice hearts, the coronary artery structure can be visualized in detail throughout the entire heart. This is done with light-sheet microscopy, a technology for generating image stacks that can be used to 3D render the arterial map. Six postnatal and juvenile timepoints were analyzed to address whether postnatal remodeling is an acute or gradual event. The resulting image stacks were mapped by a semi-automated method that yields 3D models of the coronary artery tree at each timepoint. With this experimental strategy, we hypothesize to get a holistic view of coronary arteries through different postnatal stages, pinpointing critical timepoints in which remodeling occurs. Additionally, to correlate arterial tree complexity to cardiac tissue growth, volumetric myocardial measurements were related to branch density. Understanding the mechanisms of normal postnatal coronary artery remodeling could inform the development of therapeutic manipulations aimed at resupplying blood to hypoxic areas of damaged hearts.
Description
| Type of resource | text |
|---|---|
| Date created | June 2021 |
Creators/Contributors
| Author | Sy-Quia, Ana Natalia |
|---|---|
| Primary advisor | Red-Horse, Kristy |
| Advisor | Heller, Stefan |
| Degree granting institution | Stanford University, Department of Biology, 2021 |
Subjects
| Subject | Biology |
|---|---|
| Subject | Developmental Biology |
| Subject | Coronary Artery Development |
| Subject | Arterial Remodeling |
| Genre | Thesis |
Bibliographic information
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- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
Preferred citation
- Preferred Citation
Sy-Quia, Ana Natalia; Red-Horse, Kristy; Heller, Stefan. (2021).
Characterizing how coronary arteries expand after embryonic development:
the Postnatal Wave of Arterial Remodeling. Stanford Digital Repository. Available at: https://purl.stanford.edu/nw668zr3387
Collection
Undergraduate Theses, Department of Biology, 2020-2021
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- Contact
- alsyquia@stanford.edu
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