Macroporous hydrogel sponges for enhancing stem cell transplantation and skeletal muscle regeneration following traumatic injury
Abstract/Contents
- Abstract
- Volumetric muscle loss (VML), characterized by an irreversible loss of skeletal muscle due to trauma or surgery, is accompanied by severe functional impairment and long-term disability. Tissue engineering strategies combining stem cells and biomaterials hold great promise for skeletal muscle regeneration. However, scaffolds, including decellularized extracellular matrix, hydrogels, and electrospun fibers, used for VML applications generally lack macroporosity. As a result, the aforementioned scaffolds typically delay host cell infiltration, transplanted cell proliferation, and new tissue formation. In this dissertation, I will present our work on engineering macroporous hydrogel scaffolds to enhance stem cell treatment of VML. We show that scaffold macroporosity improved muscle stem cell proliferation in vitro and in vivo. In addition, scaffold macroporosity promoted early scaffold cellularization, endothelialization, and establishment of a pro-regenerative immune microenvironment in a mouse VML model. Moreover, we found that the macroporous hydrogels enhanced muscle tissue regeneration and recovery of muscle function four weeks after implantation. Finally, we demonstrated the scalability of our approach, with respect to scaffold fabrication and therapeutic efficacy, using a rat VML model. Together, our results validate macroporous hydrogels as novel scaffolds for VML treatment and skeletal muscle regeneration.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Eugenis, Ioannis |
|---|---|
| Degree supervisor | Rando, Thomas A |
| Thesis advisor | Rando, Thomas A |
| Thesis advisor | Chaudhuri, Ovijit |
| Thesis advisor | Cochran, Jennifer R |
| Thesis advisor | Heilshorn, Sarah |
| Degree committee member | Chaudhuri, Ovijit |
| Degree committee member | Cochran, Jennifer R |
| Degree committee member | Heilshorn, Sarah |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Ioannis Eugenis. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/nt605yz3366 |
Access conditions
- Copyright
- © 2022 by Ioannis Eugenis
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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