Control of DNA capture by nanofluidic transistors
Abstract/Contents
- Abstract
- For decades, scaling of semiconductor devices has allowed massive parallelization and faster devices for higher processing throughput at a lower cost. Similar benefits can be reaped for biotechnological systems. Miniaturized microfluidic sensors and actuators have dramatically improved the throughput of biomolecular analysis by parallelization. The development of further miniaturized biotechnological sensors and actuators (in the nanoscale) is a thriving field of research today. A promising group of such nanofluidic devices are based on nanopores. Over the past 15 years nanopores have garnered significant interest as single-molecule analytical tools, particularly for DNA sequencing, and actuators to manipulate ionic and biomolecular transport. In this work, we report the use of electrically-gated ~200 nm pores as a reversible, electronically-tuneable biomolecular switch, namely, a nanofluidic transistor (NFT). We demonstrate highly effective electrostatic control of the nucleic acid capture rate with > 1000-fold modulation using sub-1V gate biases. These devices were fabricated to exploit the barrier-limited operation arising from the balanced interplay between electroosmotic flow (EOF) and electrophoresis for pores of this size and aspect ratio. The method relies on varying the gate voltage to modulate the shape of the electric double layer (EDL) to finely tune the strength of the EOF opposing the DNA's electrophoretic motion. We have determined that operating these NFTs within the sub- to near-threshold regime allows for exponential (or super-linear) control of the DNA capture rate. We present detailed numerical simulations to quantitatively elucidate the underlying mechanism of NFT operation and the effects of electrical biases, solution pHs, NFTs surface properties and NFT device dimensions. This dissertation will contain an overview of electrokinetics of nanopores, a survey of nanofluidic device research, the specifics of nanofluidic transistor fabrication used, the details of instrument development and behavior, the results of the DNA capture rate modulation experiments, and potential applications of the nanofluidic transistors studied.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2012 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Paik, Kee-Hyun |
|---|---|
| Associated with | Stanford University, Department of Electrical Engineering |
| Primary advisor | Davis, Ronald W. (Ronald Wayne), 1941- |
| Primary advisor | Dutton, Robert W |
| Thesis advisor | Davis, Ronald W. (Ronald Wayne), 1941- |
| Thesis advisor | Dutton, Robert W |
| Thesis advisor | Howe, Roger Thomas |
| Advisor | Howe, Roger Thomas |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Kee-Hyun Paik. |
|---|---|
| Note | Submitted to the Department of Electrical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2012. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2012 by Kee-Hyun Paik
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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