Modulating myoblast differentiation with RNA controllers
Abstract/Contents
- Abstract
- RNA synthetic biology utilizes the key roles of RNA in cellular information transmission and regulation to design and build new biological systems. Engineered RNA devices have been applied to many spheres of biotechnology and human health. One area of human health particularly suited to RNA device applications is regenerative medicine and tissue engineering. Skeletal muscle offers a convenient model system to test RNA device activity through the regenerative capacity of muscle mediated by muscle stem cells and their myoblast progeny. Here, I present my work to design, build, and test RNA devices for the modulation of myoblast differentiation in skeletal muscle. I first discuss the history and current state of synthetic biology, the set of possibilities regenerative medicine offers as an application space, and the latest applications of synthetic biology to mammalian cell fate decisions. Next, I review the field of engineered RNA devices and highlight the ongoing application of RNA devices as well as advances in the development of new RNA aptamers and new mechanisms of cell control using RNA. I then introduce my thesis project where I applied ribozyme-based RNA devices to myoblast differentiation and demonstrated ligand-responsive inhibition of differentiation marker transcript levels as well as inhibition of the downstream fusion of myoblasts into differentiated myotubes. Finally, I offer reflections on my project and suggestions for the design of future RNA-based regulatory systems.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Dykstra, Peter Bruce |
|---|---|
| Degree supervisor | Rando, Thomas A |
| Degree supervisor | Smolke, Christina D |
| Thesis advisor | Rando, Thomas A |
| Thesis advisor | Smolke, Christina D |
| Thesis advisor | Delp, Scott |
| Degree committee member | Delp, Scott |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Peter Bruce Dykstra. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/np650nv0700 |
Access conditions
- Copyright
- © 2022 by Peter Bruce Dykstra
- License
- This work is licensed under a Creative Commons Attribution Non Commercial No Derivatives 3.0 Unported license (CC BY-NC-ND).
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