Making waves! Acoustic and nanophotonic platforms for amplification-free cellular and molecular diagnostics
Abstract/Contents
- Abstract
- Infectious diseases are one of the leading causes of death in the 21st century, claiming the lives of over 16 million individuals each year. These staggering numbers highlight the need for rapid, sensitive, and multiplexed diagnostics to minimize the spread of infectious disease and guide appropriate treatment. Existing diagnostic methods often suffer from a need for sample culturing or molecular amplification, leading to long diagnostic times or high limits of detection when amplification is not possible. Here, I combine acoustic bioprinting with nanophotonics for 1) culture-free detection and identification of bacteria from a blood sample using surface enhanced Raman Spectroscopy and 2) amplification-free, multiplexed detection of proteins using high-Q metasurfaces. First, I describe how acoustic bioprinting combined with surface enhanced Raman spectroscopy (SERS) can be used to rapidly detect and identify pathogens from complex multicellular samples. Next, I describe a high quality-factor ("high-Q") dielectric metasurface assay that, when coupled with our home-built acoustic bioprinter for site-specific surface chemistry, allows for amplification-free detection of proteins down to femtomolar concentrations. Together, my work provides a foundation for faster, higher-throughput, and more sensitive cellular and molecular diagnostics.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Safir, Fareeha |
|---|---|
| Degree supervisor | Dionne, Jennifer Anne |
| Degree supervisor | Tang, Sindy (Sindy K.Y.) |
| Thesis advisor | Dionne, Jennifer Anne |
| Thesis advisor | Tang, Sindy (Sindy K.Y.) |
| Thesis advisor | Khuri-Yakub, Butrus T, 1948- |
| Degree committee member | Khuri-Yakub, Butrus T, 1948- |
| Associated with | Stanford University, School of Engineering |
| Associated with | Stanford University, Department of Mechanical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Fareeha Safir. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/nn586kk7686 |
Access conditions
- Copyright
- © 2023 by Fareeha Safir
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
Also listed in
Loading usage metrics...