Development and characterization of small molecule responsive RNA switches
Abstract/Contents
- Abstract
- RNA is more than a messenger -- advances in the last decade have shown RNA playing a key role beyond the central dogma in cellular and organismal regulation. Inspired by these breakthroughs researchers have developed a new generation of RNA-based devices for use in manufacturing, human health and basic research. In the span of two years mRNA vaccines were designed, tested, manufactured and distributed to hundreds of millions of humans worldwide. Improving RNA technologies requires the creation of new RNA sensors, specifically for small molecules. My thesis work covers two approaches to help improve our development of small molecule RNA sensors. We built on previous studies from our lab that detailed a novel method, DRIVER, for the enrichment of a large number small-molecular RNA aptamers. Through our work we ran a selection against 5,120 small molecules and developed new RNA aptamers against over 200 small molecules, tripling the number of small molecular RNA aptamers. Additionally, using chemical mapping techniques we characterized RNA devices developed in our lab to understand tertiary RNA-ligand interactions and improve our selection methods.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Kaplan, Matias |
|---|---|
| Degree supervisor | Bryant, Zev David |
| Degree supervisor | Smolke, Christina D |
| Thesis advisor | Bryant, Zev David |
| Thesis advisor | Smolke, Christina D |
| Thesis advisor | Fire, Andrew Zachary |
| Thesis advisor | Fordyce, Polly |
| Degree committee member | Fire, Andrew Zachary |
| Degree committee member | Fordyce, Polly |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Matias Kaplan. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/nn010yv6307 |
Access conditions
- Copyright
- © 2022 by Matias Kaplan
- License
- This work is licensed under a Creative Commons Attribution Share Alike 3.0 Unported license (CC BY-SA).
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