Designing nanoprobes for scalable electrophysiology and curvature sensing

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The advancement of nanotechnology-driven platforms has introduced numerous promis- ing biological applications in cells. Vertical nanoprobes, capable of detecting molecules and bioactive signals without labels in real-time and with high sensitivity, have become valuable tools in electrophysiology and mechanobiology. This thesis explores the diverse applications of vertical nanoprobes. The first part of this thesis focuses on nanoelectrode arrays, particularly their role in ad- dressing a significant challenge in pharmaceutical development: drug-induced cardiotoxicity. We introduce vertically-aligned nanocrown electrodes, an innovative technology with a high success rate for recording intracellular action potentials (iAPs). This breakthrough enables reliable assessment of drug-induced arrhythmogenicity. Using nanocrown electrode arrays, we can conduct precise parallel iAP recordings, facilitating comprehensive comparisons of cellular responses before and after exposure to pharmaceutical agents, all within a single cell. The latter part of our exploration delves into mechanobiology, where we demonstrate the applicability of the NanoCurvS platform for quantitatively analyzing curvature-sensitive proteins. This platform sheds light on how these proteins behave when faced with the challenges of low curvature, providing valuable insights into membrane curvature biology. These advancements represent significant progress in biomedical research, offering the potential for safer drug development and a deeper understanding of complex cellular processes. The integration of nanotechnology into electrophysiology and mechanobiology opens up new opportunities for innovative solutions and discoveries.


Type of resource text
Form electronic resource; remote; computer; online resource
Extent 1 online resource.
Place California
Place [Stanford, California]
Publisher [Stanford University]
Copyright date 2023; ©2023
Publication date 2023; 2023
Issuance monographic
Language English


Author Tsai, Ching-Ting
Degree supervisor Cui, Bianxiao
Thesis advisor Cui, Bianxiao
Thesis advisor Moerner, W. E. (William Esco), 1953-
Thesis advisor Wu, Joseph Ching-Ming, 1971-
Degree committee member Moerner, W. E. (William Esco), 1953-
Degree committee member Wu, Joseph Ching-Ming, 1971-
Associated with Stanford University, School of Humanities and Sciences
Associated with Stanford University, Department of Chemistry


Genre Theses
Genre Text

Bibliographic information

Statement of responsibility Ching-Ting Tsai.
Note Submitted to the Department of Chemistry.
Thesis Thesis Ph.D. Stanford University 2023.

Access conditions

© 2023 by Ching Ting Tsai

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