Stem cell transcriptomics : a systems approach to the pluripotent state
Abstract/Contents
- Abstract
- The discovery and isolation of human embryonic stem cells (hESCs), and the more recent generation of induced pluripotent stem cells (iPSCs) from adult cells, has given medical science the tantalizing prospect of one day regenerating organs and tissues in human patients, as well as a revolutionary method for investigating heritable human diseases in a petri dish. This is because hESCs and iPSCs are pluripotent, which enables them to differentiate into virtually any cell type of the human body. However, forcing these cells to change their phenotype is an imperfect science, and is often time-consuming, resource-intensive, and plagued by poor yields. My work over the past four years has therefore attempted to characterize the sets of molecules, both messenger RNAs and microRNAs, that together regulate pluripotency. This has included understanding how the stem cell "transcriptome" changes in the face of external insult (e.g. ionizing radiation), how it changes during differentiation to adult phenotypes such as cardiomyocytes and endothelial cells, and how knowledge from it may be used to induce pluripotency. Taken together, interrogating and ultimately controlling the stem cell transcriptome will be an essential step before we can realize the promise of regenerative therapy.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Wilson, Kitchener Daniel |
|---|---|
| Associated with | Stanford University, Department of Bioengineering. |
| Primary advisor | Gambhir, Sanjiv Sam |
| Primary advisor | Wu, Joseph Ching-Ming, 1971- |
| Thesis advisor | Gambhir, Sanjiv Sam |
| Thesis advisor | Wu, Joseph Ching-Ming, 1971- |
| Thesis advisor | Longaker, Michael T |
| Thesis advisor | Yock, Paul G |
| Advisor | Longaker, Michael T |
| Advisor | Yock, Paul G |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Kitchener Daniel Wilson. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis (Ph. D.)--Stanford University, 2010. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Kitchener Daniel Wilson
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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