Ultrafast multifocal two-photon microscopy for brain imaging
Abstract/Contents
- Abstract
- Two-photon fluorescence microscopy has extensive application in biological studies, but it is typically limited by low full-field frame rates of ∼10-20 Hz. We present a novel multifocal two-photon microscope design that can image 455 × 110 μm2 at 1 kHz frame rate and 455 × 306 μm2 at 400 Hz with micron-level resolution. We utilize an ultrashort-pulsed regenerative fiber laser amplifier as the excitation source, and use a micro-lens array to split the initial laser beam into a rectangular array of beamlets. The array of beamlets is tilted at a fixed angle along the major optical axis in the excitation path, and is scanned laterally along a single dimension with a laser-scanning mirror to cover the entire FOV at the x-y focal plane. We use a low-read-noise scientific CMOS camera to acquire multi-pixel images in the collection path of the microscope. We further demonstrate its ability to track single cell flow trajectories in cerebral arterial networks, capture cerebral bridging vein constriction events near the superior sagittal sinus, and estimate Purkinje cell Ca2+ spike timing, all with millisecond temporal resolution.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Zhang, Tong |
|---|---|
| Associated with | Stanford University, Department of Mechanical Engineering. |
| Primary advisor | Schnitzer, Mark Jacob, 1970- |
| Thesis advisor | Schnitzer, Mark Jacob, 1970- |
| Thesis advisor | Carryer, J. Edward |
| Thesis advisor | Delp, Scott |
| Advisor | Carryer, J. Edward |
| Advisor | Delp, Scott |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Tong Zhang. |
|---|---|
| Note | Submitted to the Department of Mechanical Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2015. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2015 by Tong Zhang
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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