Roles for hedgehog signaling in zebrafish development
Abstract/Contents
- Abstract
- The zebrafish has emerged as a powerful model organism for the experimental interrogation of the processes that bring about vertebrate development. In this thesis we study functions of Hedgehog (Hh) signaling, a process that is frequently dysregulated in cancer, that occur during the formation of the zebrafish embryo. First, we investigate a possible link between Hh signaling and the directed migration of germ cells to the presumptive gonad in the early embryo and find that Hh-targeting drugs can perturb germ cell migration, but Hh signaling itself does not regulate this process. Second, we study the formation of motoneurons in the developing ventral spinal cord and find that Hh signaling and retinoic acid signaling work together to induce these cells by the same downstream output of Gli transcription factor activity. Third, we generated transgenic zebrafish that reliably report Hh signaling events at multiple levels of the pathway and we apply these tools in initial studies of zebrafish regeneration. In summary, these studies further our knowledge of the roles for Hh signaling in vertebrate development and will lead insight into the pathology of Hh-related disorders.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Mich, John Kenneth |
|---|---|
| Associated with | Stanford University, Department of Biochemistry |
| Primary advisor | Chen, James Kenneth |
| Thesis advisor | Chen, James Kenneth |
| Thesis advisor | Harbury, Pehr |
| Thesis advisor | Krasnow, Mark, 1956- |
| Thesis advisor | Straight, Aaron, 1966- |
| Advisor | Harbury, Pehr |
| Advisor | Krasnow, Mark, 1956- |
| Advisor | Straight, Aaron, 1966- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | John Kenneth Mich. |
|---|---|
| Note | Submitted to the Department of Biochemistry. |
| Thesis | Ph.D. Stanford University 2010 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by John Kenneth Mich
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