Illuminating glycoconjugate interactions and glycosyltransferase substrates using photocrosslinking sugar analogs
Abstract/Contents
- Abstract
- The cell surface is composed of a heterogeneous array of proteins and lipids, many of which are modified by carbohydrate structures. Glycoconjugates, which include both glycoproteins and glycolipids, play vital roles in cellular functions, mediating communication with the extracellular environment. A frequent modification to cell surface carbohydrates is the addition of a nine-carbon [alpha]-keto acid, sialic acid. Sialic acid-dependent interactions are often characterized by large equilibrium dissociation constants and rapid dissociation rates, precluding the use of traditional biological techniques for isolating these complexes. To investigate glycan-mediated interactions, I employed the use of metabolic oligosaccharide engineering to introduce a panel of diazirine-modified sialic acids onto the cell surface. I demonstrate that the sialic acid analogs covalently trap both glycoprotein- and glycolipid-mediated interactions, illustrating the utility of the photoactivatable sugars. These results are significant as they yield a new set of tools for studying ephemeral interactions that are otherwise inaccessible.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Bond, Michelle Rueffer |
|---|---|
| Associated with | Stanford University, Department of Chemistry |
| Primary advisor | Kohler, Jennifer, 1972- |
| Primary advisor | Kool, Eric T |
| Thesis advisor | Kohler, Jennifer, 1972- |
| Thesis advisor | Kool, Eric T |
| Thesis advisor | Harbury, Pehr |
| Advisor | Harbury, Pehr |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Michelle Rueffer Bond. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2010. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Michelle Rueffer Bond
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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