High-speed isotropic resolution and structured illumination using phased arrays in light sheet fluorescence microscopy

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This dissertation details the use of high-speed, electrostatically actuated, linear phased arrays of microelectromechanical mirrors for applications in light sheet fluorescence microscopy. First, a phased array is used to demonstrate optical sectioning structured illumination to provide up to two orders of background reduction while maintaining camera-limited frame rates. The illumination is tunable, enabling the user to optimize the structured illumination performance. We then show how to use the phased array for diffraction-limited cylindrical lensing with 90% optical efficiency up to 0.008 numerical aperture (NA) with simultaneous beam steering. Single focus scanning is demonstrated up to a NA of 0.013, at which point the focus splits, creating a multifocal response. Using this lensing method, we show an axially-swept light sheet fluorescence microscope. The phased array allows camera-limited speeds without sacrificing resolution. The narrow focus of the light sheet is synchronized to the rolling shutter of a modern sCMOS camera, enabling isotropic resolution down to 720 ± 55 nm. With an update rates of 2.85 μs, the phased array can track the camera sensor rolling shutter at camera-limited rates at full frame and any reduced the region of interest, which we demonstrate at speeds of 140 frames per second at sub-micron isotropic resolution in GFP-labeled Drosophila.


Type of resource text
Form electronic resource; remote; computer; online resource
Extent 1 online resource.
Place California
Place [Stanford, California]
Publisher [Stanford University]
Copyright date 2019; ©2019
Publication date 2019; 2019
Issuance monographic
Language English


Author Landry, Joseph Russell
Degree supervisor Solgaard, Olav
Thesis advisor Solgaard, Olav
Thesis advisor Contag, Christopher H
Thesis advisor Fan, Jonathan Albert
Degree committee member Contag, Christopher H
Degree committee member Fan, Jonathan Albert
Associated with Stanford University, Department of Electrical Engineering.


Genre Theses
Genre Text

Bibliographic information

Statement of responsibility Joseph Landry.
Note Submitted to the Department of Electrical Engineering.
Thesis Thesis Ph.D. Stanford University 2019.
Location electronic resource

Access conditions

© 2019 by Joseph Russell Landry

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