High-speed isotropic resolution and structured illumination using phased arrays in light sheet fluorescence microscopy
Abstract/Contents
- Abstract
- This dissertation details the use of high-speed, electrostatically actuated, linear phased arrays of microelectromechanical mirrors for applications in light sheet fluorescence microscopy. First, a phased array is used to demonstrate optical sectioning structured illumination to provide up to two orders of background reduction while maintaining camera-limited frame rates. The illumination is tunable, enabling the user to optimize the structured illumination performance. We then show how to use the phased array for diffraction-limited cylindrical lensing with 90% optical efficiency up to 0.008 numerical aperture (NA) with simultaneous beam steering. Single focus scanning is demonstrated up to a NA of 0.013, at which point the focus splits, creating a multifocal response. Using this lensing method, we show an axially-swept light sheet fluorescence microscope. The phased array allows camera-limited speeds without sacrificing resolution. The narrow focus of the light sheet is synchronized to the rolling shutter of a modern sCMOS camera, enabling isotropic resolution down to 720 ± 55 nm. With an update rates of 2.85 μs, the phased array can track the camera sensor rolling shutter at camera-limited rates at full frame and any reduced the region of interest, which we demonstrate at speeds of 140 frames per second at sub-micron isotropic resolution in GFP-labeled Drosophila.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Landry, Joseph Russell |
|---|---|
| Degree supervisor | Solgaard, Olav |
| Thesis advisor | Solgaard, Olav |
| Thesis advisor | Contag, Christopher H |
| Thesis advisor | Fan, Jonathan Albert |
| Degree committee member | Contag, Christopher H |
| Degree committee member | Fan, Jonathan Albert |
| Associated with | Stanford University, Department of Electrical Engineering. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Joseph Landry. |
|---|---|
| Note | Submitted to the Department of Electrical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Joseph Russell Landry
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