Methods for quantiative dissection of gene regulation
Abstract/Contents
- Abstract
- Defined regulatory programs effecting diverse cellular processes such as chromatin accessibility, RNA localization or protein modifications largely determine the establishment and maintenance of a cell's identity. Here, we describe the development of quantitative and high-throughput methods for understanding principles of gene regulation. In this work, we repurpose a high-throughput sequencing instrument to develop a platform for high-throughput biochemical assays of RNA. We then develop a method for profiling chromatin accessibility within rare cellular populations or single-cells, called Assay for Transposase Accessible Chromatin with sequencing (ATAC-seq). Furthermore, we apply ATAC-seq to measure chromatin accessibility within defined cellular populations across the human hematopoietic hierarchy and primary Acute Myeloid Leukemia (AML) in effort to determine regulatory principles governing normal hematopoiesis and leukemogenesis. Together, we demonstrate that these methods provide unique insights into the molecular effectors governing normal human development and disease.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Copyright date | 2016 |
| Publication date | 2015, c2016; 2015 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Buenrostro, Jason |
|---|---|
| Associated with | Stanford University, Department of Genetics. |
| Primary advisor | Chang, Howard |
| Primary advisor | Greenleaf, William James |
| Thesis advisor | Chang, Howard |
| Thesis advisor | Greenleaf, William James |
| Thesis advisor | Crabtree, Gerald R |
| Thesis advisor | Li, Jin |
| Thesis advisor | Snyder, Michael, Ph. D |
| Advisor | Crabtree, Gerald R |
| Advisor | Li, Jin |
| Advisor | Snyder, Michael, Ph. D |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Jason Buenrostro. |
|---|---|
| Note | Submitted to the Department of Genetics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Jason Daniel Buenrostro
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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