Studies of bud-site selection in saccharomyces cerevisiae
Abstract/Contents
- Abstract
- This dissertation describes investigations of the machinery of cell polarization in the budding yeast Saccharomyces cerevisiae. Cell polarization is an essential feature of most, if not all, cell types. It occurs in two steps: (1) the selection of an axis and (2) the asymmetric organization of organelles, the cytoskeleton, and plasma-membrane components along this axis. The selection of an axis in S. cerevisiae is manifested as bud-site selection. S. cerevisiae cells select bud sites in either of two patterns, known as the axial and bipolar, depending on the mating type of the cells. Normal haploid cells use the axial pattern, whereas normal diploid cells use the bipolar pattern. Beginning with a re-analysis of the phenotypes of 38 mutants reported in a genome-wide screen to be defective in bipolar-bud-site selection, I focused on two groups of mutants with distinct phenotypes and tried to elucidate the molecular mechanisms underlying these phenotypes. The first group contains two mutants, bud13[Delta] and ist3[Delta]; the second group contains 14 endocytosis-related mutants. Each group has led to a separate story of how the corresponding proteins are involved in bipolar-bud-site selection. First, I found (Chapter 2) that Bud13 and Ist3 are responsible specifically for the efficient pre-mRNA splicing of MATa1, which encodes a cell-type-specific regulator for the expression of haploid-specific genes. One of these genes, AXL1, encodes a protein whose ectopic expression in diploid cells causes override of the bipolar-budding mechanism by the axial budding mechanism. Second, I found (Chapter 3) that endocytosis-related proteins are involved in maintaining the localization, and thus the function, of bipolar marker proteins including Bud8 and Bud9.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Tuo, Shanshan |
|---|---|
| Associated with | Stanford University, Department of Genetics. |
| Primary advisor | Pringle, John |
| Thesis advisor | Pringle, John |
| Thesis advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Sherlock, Gavin |
| Thesis advisor | Stearns, Tim |
| Advisor | Cyert, Martha S, 1958- |
| Advisor | Sherlock, Gavin |
| Advisor | Stearns, Tim |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Shanshan Tuo. |
|---|---|
| Note | Submitted to the Department of Genetics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Shanshan Tuo
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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