Developing upward going voltage indicators for general usage amongst common imaging setups in neuroscience
Abstract/Contents
- Abstract
- We have known about the electrical nature of the brain for over 100 years, and yet recording it across cells in live animals is still a work in progress. The best recording techniques for in vivo usage either track calcium concentrations with fluorescent genetically encoded calcium indicators (GECIs), or they track voltage from many cells at a single extracellular point in the cell from an extracellular electrode array. Only voltage indicators promise to record all electrical activity across the full spatial extent of all cells, but voltage sensitive dyes have not worked well in vivo, and genetically encoded voltage indicators (GEVIs) have been plagued with difficulties since their inception in 1997. Recently, we developed the ASAP4 series of GEVIs, which increase in fluorescence with depolarizing voltage, unlike previous ASAP indicators which decreased in fluorescence with depolarizing voltage. Two variants, ASAP4b and ASAP4e, feature 128% and 178% fluorescence increases over 100-mV of depolarization, respectively, and make spike detection in single trials in vivo with standard 1 and 2-photon imaging systems possible. We also perform simultaneous voltage and calcium imaging to confirm the temporal resolution and spike discernment by ASAP4 GEVIs. By creating a GEVI that uses similar equipment as calcium imaging, while providing higher temporal resolution, we hope to open up a new avenue of research to all neuroscience imaging labs.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2021; 2021 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Evans, Stephen Wenceslao |
|---|---|
| Degree supervisor | Ding, Jun (Jun B.) |
| Degree supervisor | Lin, Michael Z |
| Thesis advisor | Ding, Jun (Jun B.) |
| Thesis advisor | Lin, Michael Z |
| Thesis advisor | Giocomo, Lisa |
| Thesis advisor | Huguenard, John |
| Thesis advisor | Raymond, Jennifer L |
| Degree committee member | Giocomo, Lisa |
| Degree committee member | Huguenard, John |
| Degree committee member | Raymond, Jennifer L |
| Associated with | Stanford University, Neurosciences Program |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Stephen Wenceslao Evans. |
|---|---|
| Note | Submitted to the Neurosciences Program. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/mk150td1401 |
Access conditions
- Copyright
- © 2021 by Stephen Wenceslao Evans
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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