3D human liver tissue engineering for hepatic diseases study and cell replacement therapies
Abstract/Contents
- Abstract
- Engineering of 3D human liver tissue is important to model authentic human liver for drug testing, disease modeling, and transplantable construct development that can be an alternative solution for human liver transplantation which is the only reliable option for end-stage liver diseases but suffers from a human donor shortage. Recently, hydrogel inverted colloidal crystal (ICC) scaffold-based liver systems were introduced as mature functional liver constructs with human fetal liver cells and human induced pluripotent stem cells (iPS)-derived hepatocytes. However, primary human adult hepatocytes (hHeps), the gold standard cell type for human liver tissue engineering, has not yet been introduced to the ICC system, thus limiting multiple applications requiring highly preserved liver-specific functions and highly authentic liver modeling such as phase II metabolism, hepatitis B and D infection, fatty liver modeling and IL-6 release modeling for finding new drug targets, novel candidate drug screening and novel strategies for transplanting engineered human liver tissues. In this thesis, I developed hHep-based authentic human liver systems using several types of advanced ICC scaffolds and characterized authentic human liver functions that were not explored in other ICC systems before. Using the developed systems, human liver diseases were modeled, and we explored a new drug target for combatting hepatitis B infection and screened novel drug candidates for fatty liver disease, hepatitis D infection, and cytokine storms, which can be promising future therapeutics. Also, the system was applied to transplantation studies, and it showed proof-of-concept potential to be a bridge or an alternative to current human liver transplantation.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2021; 2021 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | No, Da Yoon |
|---|---|
| Degree supervisor | Glenn, Jeffrey S, 1962- |
| Thesis advisor | Glenn, Jeffrey S, 1962- |
| Thesis advisor | Esquivel, Carlos |
| Thesis advisor | Frank, C. W |
| Thesis advisor | Yang, Fan, (Bioengineering researcher and teacher) |
| Degree committee member | Esquivel, Carlos |
| Degree committee member | Frank, C. W |
| Degree committee member | Yang, Fan, (Bioengineering researcher and teacher) |
| Associated with | Stanford University, Department of Bioengineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Da Yoon No. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/mh507vd7788 |
Access conditions
- Copyright
- © 2021 by Da Yoon No
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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