Plasmonic gold nanomaterials for medical diagnosis and biological imaging
- This thesis describes the development, optimization and application of plasmonic gold nanomaterials in medical diagnosis and biological imaging. Gold nanoparticles undergo unique localized surface plasmon resonances in the visible and near-infrared (NIR). Coupling of the scattering nanoparticle plasmon modes result in enhanced local electric field and NIR fluorescence emission rate, which can lead to NIR fluorescence enhancement (NIR-FE) under certain conditions. Here, the application of this NIR-FE effect is discussed in the context of protein microarray immunoassays. Multiplexed protein assays are constructed on the nanostructured plasmonic gold film that display up to 100-fold fluorescence enhancement and ~3 orders of magnitude extension of the protein detection dynamic range, compared to conventional assays on commercial substrates such as glass. Immunoassays performed on the plasmonic gold films afford more sensitive measurements of proteins over a broader dynamic range with higher signal-to-noise ratio. The aim of this thesis is to develop novel protein microarray immunoassays for biomedical diagnostics on the plasmonic gold platform to conquer some of the most challenging healthcare problems faced by global population. The clinical application of plasmonic substrates is demonstrated from the diagnosis of infectious diseases and the early detection of hypertensive heart diseases. The application of the plasmonic gold nanomaterials for sensitive imaging and detection of cellular proteins is also presented.
|Type of resource
|electronic; electronic resource; remote
|1 online resource.
|Stanford University, Department of Chemistry.
|Dai, Hongjie, 1966-
|Dai, Hongjie, 1966-
|Statement of responsibility
|Submitted to the Department of Chemistry.
|Thesis (Ph.D.)--Stanford University, 2017.
- © 2017 by Xiaoyang Li
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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