Materials and methods for in vitro electrophysiological measurement
Abstract/Contents
- Abstract
- The pace of progress in measuring electrical phenomena in cells and tissues has largely been dictated by the available measurement tools. These tools can be grouped into two general classes: those that measure the intracellular potential of cells and those that measure changes of the membrane potential extracellularly. In this thesis, I cover the development of three in vitro tools across the two classes of measurement. For intracellular measurement of electrophysiology, I discuss the fabrication and optimization of nanopillar electrodes. These nanopillars, when coupled with electroporation, temporarily yield low impedance access to the intracellular potential. In fabrication, I show a method which uses two lithography steps and no dry etches to generate high aspect ratio electrodes. In optimization, I explore parameters of electroporation which yield the highest amplitude and longest duration intracellular recordings from stem cell-derived cardiomyocytes. For extracellular measurement, I introduce a new motif in recording two-dimensional areas with a single amplification path and with neither dead space nor electrical cross-talk. This motif is enabled by the optoelectronic properties of graphene. In our configuration, a change in the electrostatic gating of graphene yields a change in its absorptivity which we exploit to transduce the electrical spikes of cells into optical signals. I first detail the voltaic and temporal resolution of the system. I then demonstrate the application of this technique to the recording of field potentials from an embryonic chicken heart. In the final tool, I demonstrate the use of soft three-dimensional electrodes to minimally invasively record field potentials from cardiac cells.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2018; 2018 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | McGuire, Allister Francis |
|---|---|
| Degree supervisor | Cui, Bianxiao |
| Thesis advisor | Cui, Bianxiao |
| Thesis advisor | Boxer, Steven G. (Steven George), 1947- |
| Thesis advisor | Cui, Yi, 1976- |
| Degree committee member | Boxer, Steven G. (Steven George), 1947- |
| Degree committee member | Cui, Yi, 1976- |
| Associated with | Stanford University, Department of Chemistry. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Allister Francis McGuire. |
|---|---|
| Note | Submitted to the Department of Chemistry. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Allister Francis McGuire
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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