Glycogen phase separation drives macromolecular rearrangement and asymmetric division upon nutrient depletion in escherichia coli
Abstract/Contents
- Abstract
- Bacteria often experience nutrient limitation in nature and the laboratory. While the exponential and stationary phases of growth have been well characterized in the model bacterium Escherichia coli, little is known about what transpires inside individual cells during the transition between these two phases. In Chapter 2, using quantitative cell imaging along the growth curve, we found that the positions of the nucleoid and cell division become increasingly asymmetric during the transition phase. These asymmetries were coupled with a spatial reorganization of proteins, ribosomes and RNAs, which adopted a more nucleoid-centric localization. In Chapter 3 we show results from live-cell imaging experiments, complemented with genetic and 13C whole-cell nuclear magnetic resonance spectroscopy studies, that accumulation of the storage polymer glycogen at the old pole leads to the observed macromolecular rearrangement and asymmetric division. Finally in Chapter 4, using an in vitro system we present data suggesting that these phenotypes are likely due to the propensity of glycogen to phase separate into liquid condensates in crowding environments. Cell size differences between strains and daughter cells suggest that glycogen phase separation results in cytoplasmic space compensation that allows cells to store a glucose polymer in large amount without affecting macromolecular homeostasis during nutrient-limiting growth. Finally, these glycogen-dependent effects also occur during exponential phase in a cell age-dependent manner.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Thappeta, Yashna |
|---|---|
| Degree supervisor | Jacobs-Wagner, Christine |
| Thesis advisor | Jacobs-Wagner, Christine |
| Thesis advisor | Brandman, Onn |
| Thesis advisor | Cremer, Jonas |
| Thesis advisor | Huang, Kerwyn Casey, 1979- |
| Degree committee member | Brandman, Onn |
| Degree committee member | Cremer, Jonas |
| Degree committee member | Huang, Kerwyn Casey, 1979- |
| Associated with | Stanford University, School of Humanities and Sciences |
| Associated with | Stanford University, Department of Biology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Yashna Thappeta. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/kt587kk2920 |
Access conditions
- Copyright
- © 2023 by Yashna Thappeta
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