Elucidating the role of the PRC2 sub-unit Ezh2 during early xenopus development and investigating human placentation and pregnancy using first trimester chorionic villi
Abstract/Contents
- Abstract
- The Polycomb Repressive Complex 2 (PRC2) is of central importance to the establishment and maintenance of transcriptional silencing during development. Limited research on the role of PRC2 during early vertebrate development has produced very different, species-specific results, and recent studies have uncovered roles for the catalytic subunit, Ezh2, independent of its ability to tri-methylate lysine 27 of histone 3 (H3K27me3). In Chapter 1, I review recent findings on the role and regulation of Ezh2 in vertebrate models. In Chapter 2, I present my findings on the role of the Xenopus laevis ezh2 homolog during early development. Embryos deficient for ezh2 failed to specify the mesoderm and were unable to proceed successfully through gastrulation. The effects of ezh2 knockdown seemed to occur prior to the deposition of H3K27me3 in the Xenopus embryo, suggesting a non-canonical role of ezh2 during germ layer specification. Furthermore, deposition of H3K27me3 by the neurula stage was unaffected by depletion of ezh2. In Chapter 3, I present the findings from a collaboration between our laboratory and the Lucille Packard Children's Hospital studying placentation during the first trimester of human pregnancy. Chorionic villus sampling (CVS) is routinely used for prenatal diagnosis of cytogenetic disorders, but the potential of these samples to provide insights into the etiology of pregnancy complications like pre-eclampsia remains largely unexplored. After examining the morphology and transcriptomes of first trimester chorionic villi, we found that pre-eclampsia markers were associated with villi with low morphological complexity and identified the gene SVEP1 as a possible biomarker for determining gestational age.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2019; ©2019 |
| Publication date | 2019; 2019 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | González-Maldonado, Eduardo |
|---|---|
| Degree supervisor | Baker, Julie, (Professor of genetics) |
| Degree committee member | Fuller, Margaret T, 1951- |
| Degree committee member | Nusse, Roel, 1950- |
| Degree committee member | Wysocka, Joanna, Ph. D |
| Thesis advisor | Fuller, Margaret T, 1951- |
| Thesis advisor | Nusse, Roel, 1950- |
| Thesis advisor | Wysocka, Joanna, Ph. D |
| Associated with | Stanford University, Department of Developmental Biology. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Eduardo González-Maldonado. |
|---|---|
| Note | Submitted to the Department of Developmental Biology. |
| Thesis | Thesis Ph.D. Stanford University 2019. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2019 by Eduardo Gonzalez-Maldonado
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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