Molecular basis of respiratory plasticity in Shewanella oneidensis MR-1
Abstract/Contents
- Abstract
- The gamma-proteobacterium Shewanella oneidensis MR-1 utilizes a complex electron transfer network composed primarily of c-type cytochromes as well as iron-sulfur proteins, molybdoenzymes, and flavins, to respire under anoxic conditions a variety of compounds, including fumarate, nitrate, and dimethylsulfoxide (DMSO) in addition to Fe (III) and Mn (IV) minerals. Central to several respiratory pathways in this dissimilatory metal reducing bacterium (DMRB), including the electron transfer pathway to Fe (III), is CymA, a cytoplasmic membrane-bound tetraheme c-type cytochrome that functions as the major hydroquinone dehydrogenase and couples electron transport to various periplasmic oxidoreductases. Using a genetic approach involving the reconstruction of the putative electron transport chain of dissimilatory iron reduction from Shewanella oneidensis MR-1 in Escherichia coli, I showed that expression of cymA was necessary and sufficient to convert E. coli into a dissimilatory iron reducing (DIR) bacterium, indicating that CymA as a terminal reductase was the minimal electron transport chain within S. oneidensis MR-1 to Fe (III). In addition, we isolated and characterized a [delta]cymA suppressor mutant that was capable of respiration of fumarate, ferric citrate, and DMSO, but not of nitrate. The suppression was found to be due to insertion sequence-mediated transcriptional activation of mccCD, which encode for a putative periplasmic iron sulfur protein and an integral membrane hydroquinone dehydrogenase, respectively.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2010 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Cordova, Carmen Dolores |
|---|---|
| Associated with | Stanford University, Civil & Environmental Engineering Department |
| Primary advisor | Spormann, Alfred M |
| Thesis advisor | Spormann, Alfred M |
| Thesis advisor | Khosla, Chaitan, 1964- |
| Thesis advisor | Long, Sharon |
| Advisor | Khosla, Chaitan, 1964- |
| Advisor | Long, Sharon |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Carmen Dolores Cordova. |
|---|---|
| Note | Submitted to the Department of Civil and Environmental Engineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2010. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2010 by Carmen Dolores Cordova
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