PERSIST-seq in solution stability data for Stanford OpenVaccine orders 16 and 17a, unmodified and pseudouridylated nanoluciferase mRNA, 06-24-21
Abstract/Contents
- Abstract
Illumina sequencing data (FASTQ files) and associated sample sheets collected for unmodified as well as pseudouridylated nanoluciferase mRNA designs shown in Fig. 4 of the paper:
Leppek, K., Byeon, G.W., Kladwang, W. et al. Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics. Nat Commun 13, 1536 (2022). https://doi.org/10.1038/s41467-022-28776-w
These are data collected to test whether in solution lifetimes measure in the pooled format of PERSIST-seq (Pooled Evaluation of mRNA in-solution Stability, and In-cell Stability and Translation RNA-seq) match lifetimes measured in one-by-one experiments. Note that preliminary analysis suggests that the experimental pipeline results in artifactual decay curves where some of the mRNA's appear to persist longer in the pooled experiment data than in one-by-one experiments, perhaps due to RT-PCR artefacts.
Description
Type of resource | Dataset, text |
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Date created | June 24, 2021 |
Date modified | December 5, 2022 |
Publication date | May 23, 2022; May 22, 2022 |
Creators/Contributors
Author | Das, Rhiju |
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Author | Kladwang, Wipapat |
Subjects
Subject | PERSIST-seq |
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Subject | Pseudouridine |
Subject | Stability |
Subject | Vaccines |
Subject | nanoluciferase |
Subject | mRNA |
Subject | Messenger RNA |
Genre | Data |
Genre | Tabular data |
Genre | Data |
Genre | Data sets |
Genre | Dataset |
Genre | Tables (data) |
Bibliographic information
Related item |
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DOI | https://doi.org/10.25740/kb695qv0103 |
Location | https://purl.stanford.edu/kb695qv0103 |
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution 4.0 International license (CC BY).
Preferred citation
- Preferred citation
- Das, R. and Kladwang, W. (2022). PERSIST-seq in solution stability data for Stanford OpenVaccine orders 16 and 17a, unmodified and pseudouridylated nanoluciferase mRNA, 06-24-21. Stanford Digital Repository. Available at https://purl.stanford.edu/kb695qv0103
Collection
Stanford Research Data
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