Genetic probing of the origin of alveolar myofibroblasts and bronchopulmonary dysplasia, a disease of alveolar development
Abstract/Contents
- Abstract
- Alveolarization, the final stage of lung development, promotes gas-exchange by dividing the sac-like terminal airspaces of the lung into thin-walled pockets. Myofibroblasts are found at sites of septation, and mouse mutants in PDGFR alpha signaling lack myofibroblasts and air sacs fail to septate, suggesting that myofibroblasts play a role in septation. Further, a similar arrest in alveolarization is observed in diseases like bronchopulmonary dysplasia (BPD), a disease of premature infants that twin studies implicate may have important genetic contributions. In this thesis, I explore the developmental origins of myofibroblasts in mouse and the genetic basis of BPD. I first describe a novel protocol developed to extract DNA from dried blood spots taken from newborn infants that can be used in genotyping and genome-wide association studies (GWAS). I then describe an agnostic exploration of the genetic basis of BPD. Finally, I describe the use of genetic lineage tracing in mice to identify airway smooth muscle cells as a source of ductal myofibroblasts during lung development.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2013 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | St. Julien, Krystal Renee |
|---|---|
| Associated with | Stanford University, Department of Biochemistry. |
| Primary advisor | Krasnow, Mark, 1956- |
| Thesis advisor | Krasnow, Mark, 1956- |
| Thesis advisor | Brown, Patrick O'Reilly, 1954- |
| Thesis advisor | Spudich, James A |
| Advisor | Brown, Patrick O'Reilly, 1954- |
| Advisor | Spudich, James A |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Krystal Renee St. Julien. |
|---|---|
| Note | Submitted to the Department of Biochemistry. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2013. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2013 by Krystal Renee St. Julien
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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