The house of tudor : a diversity of functionally critical arginine methylation sites in endogenous RNA interference
Abstract/Contents
- Abstract
- The discovery of RNA interference (RNAi) was a doubly significant event in that it not only ushered in a new era of technical capability to specifically silence genes in a wide range of experimental contexts, but it also opened a new field of inquiry: the endogenous function of RNAi factors. Many of these functions have now been well characterized, but others remain stubbornly obscure and even controversial. In recent years, the discovery of the CRISPR bacterial and archaeal immune systems has had a dual impact on the biological sciences comparable to RNAi. Dramatically improved genome editing with CRISPR-derived techniques has been accompanied by a flurry of studies concerning the mechanism of CRISPR-based immunity. The ability to edit the genome of an organism is one of the most potent experimental tools in genetics. The rapid spread of CRISPR technology has enabled a profusion of highly precise genetic experiments in a wide variety of organisms. However, while the technology is powerful and efficient, it has limitations. A third development in the modern era that has revolutionized biology is the advent of next generation sequencing technologies. These methods make possible a large variety of assays, and in particular, they allow sequencing and characterization of small RNA populations that participate in endogenous RNAi. Here, I present my work to develop and improve CRISPR/Cas9 genome editing in Caenorhabditis elegans, as well as subsequent studies using the method, in conjunction with small RNA-seq, to elucidate the enigmatic endogenous role of RNAi.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2017 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Bell, Ryan T |
|---|---|
| Associated with | Stanford University, Department of Genetics. |
| Primary advisor | Fire, Andrew Zachary |
| Thesis advisor | Fire, Andrew Zachary |
| Thesis advisor | Brunet, Anne, 1972- |
| Thesis advisor | Kim, Stuart |
| Thesis advisor | Li, Jin |
| Advisor | Brunet, Anne, 1972- |
| Advisor | Kim, Stuart |
| Advisor | Li, Jin |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Ryan T. Bell. |
|---|---|
| Note | Submitted to the Department of Genetics. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2017. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2017 by Ryan Tierney Bell
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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