Baby's first hug: understanding the relationship between embryo biomechanical properties and developmental potential
Abstract/Contents
- Abstract
- The work covered in this dissertation represents a significant contribution to both the clinical practice of assisted reproduction and to the scientific study of embryo development. It has resulted in the development of a new technology to improve oocyte and embryo viability assessment after in vitro fertilization, which will enable physicians to make more optimal decisions about embryo transfer and improve outcomes for mothers and babies. It has also provided a new tool to scientists wishing to study the fundamental mechanisms underlying oocyte maturation and embryo development. To achieve these contributions, the following studies were performed: (1) An automated robotic device was developed which can noninvasively measure oocyte and embryo biomechanical properties. (2) The ability of oocyte and embryo biomechanics to predict developmental outcomes was validated in preclinical studies using mouse and human research embryos. These were followed by a pilot clinical trial which measured the ability of embryo biomechanics to predict clinical pregnancy in humans. (3) Factors important for successful embryo development were investigated by measuring differences in gene expression between embryos with low and high developmental potential. Experiments were also performed to understand the origin of the link between embryo mechanical phenotype and developmental potential.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Yanez, Livia Zarnescu |
|---|---|
| Associated with | Stanford University, Department of Bioengineering. |
| Primary advisor | Camarillo, David |
| Thesis advisor | Camarillo, David |
| Thesis advisor | Behr, Barry |
| Thesis advisor | Gitler, Aaron D |
| Thesis advisor | Quake, Stephen Ronald |
| Advisor | Behr, Barry |
| Advisor | Gitler, Aaron D |
| Advisor | Quake, Stephen Ronald |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Livia Zarnescu Yanez. |
|---|---|
| Note | Submitted to the Department of Bioengineering. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Livia Zarnescu Yanez
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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