Assessment and application of methodologies for the study of physiology and pathogens of the gastrointestinal tract
Abstract/Contents
- Abstract
- The gastrointestinal system is a major organ system that performs numerous functions to maintain organismal health. To maximize our ability to make discoveries about gastrointestinal health and cure gastrointestinal diseases, we must make novel insights into the factors governing gastrointestinal function as well as improve the methods by which we study the gastrointestinal tract. In this dissertation, I present three projects that detail my efforts to do both. In Chapter 1, I summarize the background and motivation for the different projects. In Chapter 2, we examine how the gut physiology of mice, a ubiquitous animal model, is altered by proposed warming of ambient housing temperature in facilities to mouse thermoneutrality (30°C) from standard facility temperatures (22°C). We report, for the first time, a temperature-dependent difference in mouse gut motility that is driven primarily by differences in stress levels experienced at 22°C and 30°C. Additionally, we report the first species-level differences in the gut microbiota of mice raised at 22°C and 30°C. In Chapter 3, we compared the efficacy of different methods for the detection of SARS-CoV-2 in fecal samples from patients with COVID-19. We ultimately recommend a rigorously benchmarked pipeline to maximize the accuracy with which researchers can study the dynamics of fecal SARS-CoV-2 RNA shedding. In Chapter 4, we apply a novel bioinformatic tool to identify insertion-enriched regions of the Salmonella Typhimurium genome that, when disrupted by mobile genetic elements, may provide antimicrobial resistance. We create deletion mutants and screen them against antimicrobials, identifying several that produce altered resistance and sensitivity profiles to numerous chemicals. Finally, in Chapter 5, I summarize the intellectual contributions of this dissertation and discuss recommended future directions for re-search to further the findings from each preceding chapter. Altogether, the work presented here advances both our insights into factors governing gastrointestinal function as well as the methods with which we can study the gastrointestinal tract.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2023; ©2023 |
| Publication date | 2023; 2023 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Han, Alvin |
|---|---|
| Degree supervisor | Bhatt, Ami (Ami Siddharth) |
| Degree supervisor | Monack, Denise M |
| Thesis advisor | Bhatt, Ami (Ami Siddharth) |
| Thesis advisor | Monack, Denise M |
| Thesis advisor | Kaltschmidt, Julia |
| Thesis advisor | Schneider, David (David Samuel) |
| Thesis advisor | Sonnenburg, Justin, 1973- |
| Degree committee member | Kaltschmidt, Julia |
| Degree committee member | Schneider, David (David Samuel) |
| Degree committee member | Sonnenburg, Justin, 1973- |
| Associated with | Stanford University, School of Medicine |
| Associated with | Stanford University, Department of Microbiology and Immunology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Alvin Han. |
|---|---|
| Note | Submitted to the Department of Microbiology and Immunology. |
| Thesis | Thesis Ph.D. Stanford University 2023. |
| Location | https://purl.stanford.edu/jj665dr4284 |
Access conditions
- Copyright
- © 2023 by Alvin Han
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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