Calcineurin regulates a Cdc42 GAP, Rga2, to modulate Cdc42 signaling during the yeast pheromone response
Abstract/Contents
- Abstract
- Rho GTPases are central regulators of cell polarity whose activity must be tightly regulated. In S. cerevisiae, the essential small type Rho GTPase Cdc42 regulates cell polarity during vegetative growth and coordinates cell signaling with morphogenesis in the mating response. Three Rho GTPase activating proteins (GAPs), Bem3, Rga1, Rga2, contribute to the specific spatial and temporal regulation of Cdc42, serving both overlapping and unique regulatory functions. Phosphorylation of these GAPs are known to regulate their function as during the G1 phase of the cell cycle, the cyclin-dependent kinases phosphorylate and inhibit Rga2 at S/TP sites to promote Cdc42 activation during bud emergence. Regulation of these phosphosites by phosphatases and other kinases remains to be examined. A recent systematic screen identified Rga2 as a substrate for the Ca2+-activated phosphatase, calcineurin. Our studies reveal that calcineurin dephosphorylates Rga2 during pheromone signaling to activate its function in limiting Cdc42 signaling. Calcineurin regulation of Rga2 is important to dampen pheromone-induced gene expression and promote cell survival during prolonged exposure to mating factor. Together with the known functions of calcineurin in promoting endocytosis of the pheromone receptor and inhibiting transcription factor activity, calcineurin signaling provides critical negative feedback during the pheromone response that is essential for cell survival.
Description
| Type of resource | text |
|---|---|
| Form | electronic; electronic resource; remote |
| Extent | 1 online resource. |
| Publication date | 2016 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Associated with | Ly, Nina |
|---|---|
| Associated with | Stanford University, Department of Biology. |
| Primary advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Nelson, William |
| Thesis advisor | Pringle, John |
| Thesis advisor | Shen, Kang, 1972- |
| Advisor | Nelson, William |
| Advisor | Pringle, John |
| Advisor | Shen, Kang, 1972- |
Subjects
| Genre | Theses |
|---|
Bibliographic information
| Statement of responsibility | Nina Ly. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis (Ph.D.)--Stanford University, 2016. |
| Location | electronic resource |
Access conditions
- Copyright
- © 2016 by Nina Ly
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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