Complex behavior enforced by protein-based inheritance : investigating the consequences of novel prion elements on sexual reproduction and genome integrity in the budding yeast Saccharomyces cerevisiae
Abstract/Contents
- Abstract
- Prions are proteins that can adopt multiple conformations, at least one of which is self-templating. Ensuing changes in protein activity are heritable over long biological timescales, in a non-Mendelian fashion. In budding yeast, specific prions have been identified that confer distinct phenotypes and fitness advantages in different conditions with induction and loss at rates higher than mutation. These prions can be induced by different stimuli -- in some cases, stimuli to which they provide an adapted response. This bi-stable switch behavior provides a framework for bet-hedging strategies whereby organisms can adapt to fluctuating environments in a reversible manner. Here, I review phase-separation dynamics and their biological relevance as they relate to and inform prion behavior (Chapter 1), followed by reports of two cellular states: [MIX+] (Chapter 2), and Ste+ (Chapter 3). [MIX+] is a prion state associated with the helicase, Mph1. It decreases mutation frequency, increases the incidence of recombination, and provides broad-spectrum resistance to a variety of DNA damaging conditions. Its induction can be stimulated by DNA-damaging conditions to which it provides resistance. Ste+ is a putative prion state associated with the Aurora B Kinase scaffold, Sli15. Ste+ cells demonstrate sterility, distinct histone modification, and a unique transcriptional profile. However, the sterility phenotype is in part explained by a nonsense mutation in STE7. Nevertheless, together, these efforts represent an effort to investigate mechanisms by which S. cerevisiae can modulate genomic integrity and diversity through switch-like control of sexual reproduction, mutation, and recombination.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2021; ©2021 |
| Publication date | 2021; 2021 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Futia, Raymond Aaron |
|---|---|
| Degree supervisor | Jarosz, Daniel |
| Thesis advisor | Jarosz, Daniel |
| Thesis advisor | Cyert, Martha S, 1958- |
| Thesis advisor | Feldman, Jessica L |
| Thesis advisor | Skotheim, Jan, 1977- |
| Thesis advisor | Straight, Aaron, 1966- |
| Degree committee member | Cyert, Martha S, 1958- |
| Degree committee member | Feldman, Jessica L |
| Degree committee member | Skotheim, Jan, 1977- |
| Degree committee member | Straight, Aaron, 1966- |
| Associated with | Stanford University, Department of Biology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Raymond A. Futia. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2021. |
| Location | https://purl.stanford.edu/hs106xk1484 |
Access conditions
- Copyright
- © 2021 by Raymond Aaron Futia
- License
- This work is licensed under a Creative Commons Attribution 3.0 Unported license (CC BY).
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