A physical and genetic interaction map of the ras pathway in lung Adenocarcinoma
Abstract/Contents
- Abstract
- Cells determine responses to their environment through the ordered formation of stable and transient molecular complexes. The mapping of intermolecular interac- tions is therefore a core task of cell and molecular biologists. This work describes a new method, QSHIP, for the analysis of affinity purification/mass spectrometry (AP/MS) data, which allows for the confident identification of protein-protein inter- actions (PPIs). It also describes the application of this and other interaction-mapping methods to the RAS pathway in lung cancer. These PPIs were used to plan CRISPR dual knockout (CDKO) experiments, which identify genetic interactions between pairs of genes. Together, these interaction maps greatly expand our knowledge of the RAS pathway in lung cancer and present a new, generalizable approach to interaction mapping in human disease
Description
| Alternative title | Proteomic and genomic mapping of the Ras pathway in lung adenocarcnioma |
|---|---|
| Type of resource | text |
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2020; ©2020 |
| Publication date | 2020; 2020 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Kelly, Marcus Robert |
|---|---|
| Degree supervisor | Jackson, Peter K. (Peter Kent) |
| Thesis advisor | Jackson, Peter K. (Peter Kent) |
| Thesis advisor | Bassik, Michael |
| Thesis advisor | Winslow, Monte |
| Degree committee member | Bassik, Michael |
| Degree committee member | Winslow, Monte |
| Associated with | Stanford University, Cancer Biology Program. |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Marcus Robert Kelly |
|---|---|
| Note | Submitted to the Cancer Biology Program |
| Thesis | Thesis Ph.D. Stanford University 2020 |
| Location | electronic resource |
Access conditions
- Copyright
- © 2020 by Marcus Robert Kelly
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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