Molecular tools for capturing transient cellular events
Abstract/Contents
- Abstract
- Transient cellular events, including their activity profile (e.g. calcium activity in neurons), their dynamic genetic background (e.g. transcriptome signature) or protein-protein interactions (PPIs) underlie important biological processes. Tools that enable the identification and labeling of cells based on these transient events are fundamental in studying and manipulating cellular behavior. In chapter 1 of this thesis, I discuss a new dual-purpose real-time calcium indicator and transcriptional calcium integrator called LuCID. LuCID's calcium-dependent split luciferase component provides real-time bioluminescence readout of calcium dynamics in cells, while its GI/FKF1 split GAL4 component converts calcium-generated bioluminescence into stable gene expression. I show that LuCID's modular design enables it to read out other cellular events such as protein-protein interactions. In chapter 2, I outline my efforts to develop a RNA detector for deploying CRISPR in a cell-type specific manner and, in Chapter 3, I outline my efforts towards developing a plate-based assay for using proximity-labeling technology to capture PPIs for high-throughput drug screens.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Erdenee, Elbegduuren |
|---|---|
| Degree supervisor | Ting, Alice Y |
| Thesis advisor | Ting, Alice Y |
| Thesis advisor | Bassik, Michael |
| Thesis advisor | Luo, Liqun, 1966- |
| Thesis advisor | Shen, Kang, 1972- |
| Degree committee member | Bassik, Michael |
| Degree committee member | Luo, Liqun, 1966- |
| Degree committee member | Shen, Kang, 1972- |
| Associated with | Stanford University, Department of Biology |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Elbegduuren Erdenee. |
|---|---|
| Note | Submitted to the Department of Biology. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/hq965fc8243 |
Access conditions
- Copyright
- © 2022 by Elbegduuren Erdenee
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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