A Mechatronic Solution for Time-Resolved Cryogenic Electron-Microscopy Sample Preparation

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Abstract/Contents

Abstract: Cryogenic Electron Microscopy (Cryo-EM) is an established method of imaging biomolecules with an electron microscope. The current process of preparing samples for Cryo-EM involves loading the grid into a plunge-freezer and plunging it into liquid ethane to freeze the grid. As the grid falls into the liquid ethane, two samples are mixed and deposited on the grid using a microfluidic spraying device. In order to improve the practicality and repeatability of the plunge-freezer, a mechatronic device was built to simplify the process of replacing the microfluidic spraying device.

Author	Di Perna, Maximus
Thesis advisor	Wakatsuki, Soichi
Thesis advisor	Howe, Roger
Degree granting institution	Stanford University
Department	Program in Engineering Physics

DOI	https://doi.org/10.25740/hq778yt7524
Location	https://purl.stanford.edu/hq778yt7524

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License: This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International license (CC BY-NC).

Preferred citation: Di Perna, M. (2023). A Mechatronic Solution for Time-Resolved Cryogenic Electron-Microscopy Sample Preparation. Stanford Digital Repository. Available at https://purl.stanford.edu/hq778yt7524. https://doi.org/10.25740/hq778yt7524.

Undergraduate Theses, Program in Engineering Physics

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