Unraveling fundamental questions of polyketide synthases through biochemical and structural studies
Abstract/Contents
- Abstract
- A good test of one's understanding of an enzyme's structure-mechanism relationships is the ability to engineer the enzyme to exhibit a new property. Through a deeper understanding of active site structures, several enzymes have been engineered however, assembly-line polyketide synthases (PKSs) have been relatively resistant to improvement via engineering. These PKSs are large multifunctional and multimodular enzymes that are responsible for the biosynthesis of many medicinally relevant compounds. The co-linear biosynthetic mechanisms of assembly-line PKSs have inspired researchers to "mix-and-match" domains and modules to make novel natural products. Unfortunately, in most cases, the yields of these new products are often very low. It is generally believed that improvements in the catalytic activities of these hybrid enzymes would depend on an improved understanding of critical protein-protein and protein-substrate interfaces and interactions. In this thesis, two PKSs (NOCAP and DEBS) were studied through biochemical and structural analysis to gain insight into fundamental questions of how these enzymes operate.
Description
| Type of resource | text |
|---|---|
| Form | electronic resource; remote; computer; online resource |
| Extent | 1 online resource. |
| Place | California |
| Place | [Stanford, California] |
| Publisher | [Stanford University] |
| Copyright date | 2022; ©2022 |
| Publication date | 2022; 2022 |
| Issuance | monographic |
| Language | English |
Creators/Contributors
| Author | Guzman, Katarina Maria |
|---|---|
| Degree supervisor | Khosla, Chaitan, 1964- |
| Thesis advisor | Khosla, Chaitan, 1964- |
| Thesis advisor | Barnes, Christopher |
| Thesis advisor | Sattely, Elizabeth |
| Degree committee member | Barnes, Christopher |
| Degree committee member | Sattely, Elizabeth |
| Associated with | Stanford University, Department of Chemical Engineering |
Subjects
| Genre | Theses |
|---|---|
| Genre | Text |
Bibliographic information
| Statement of responsibility | Katarina M. Guzman. |
|---|---|
| Note | Submitted to the Department of Chemical Engineering. |
| Thesis | Thesis Ph.D. Stanford University 2022. |
| Location | https://purl.stanford.edu/hq742pc1394 |
Access conditions
- Copyright
- © 2022 by Katarina Maria Guzman
- License
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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