Supplementary data on Eterna-designed superfolder ribosomes
Abstract/Contents
- Abstract
- Functional design of ribosomes with mutant ribosomal RNA (rRNA) could expand opportunities for understanding molecular translation, building cells from the bottom-up, and engineering ribosomes with altered capabilities. However, such efforts have been hampered by cell viability constraints, an enormous combinatorial design space, and limitations on large-scale, 3D design of RNA structures and functions. To address these challenges, we developed an integrated community science and experimental screening approach for rational design of ribosomes. This approach couples Eterna, an online video game that crowdsources RNA sequence design to community scientists in multiple design-build-test-learn cycles. We applied our framework to discover mutant rRNA sequences that improve protein synthesis in vitro and cell growth in vivo, relative to wild type ribosomes, under diverse environmental conditions. This work provides new insights into ribosome rRNA sequence-function relationships and has implications for synthetic biology.
Description
| Type of resource | Dataset, still image |
|---|---|
| Date modified | July 31, 2022; August 2, 2022; November 28, 2022; November 28, 2022; December 13, 2022 |
| Publication date | March 17, 2022 |
Creators/Contributors
| Author | Kruger, Antje |
|---|---|
| Author | Watkins, Andrew |
| Author | Wellington-Oguri, Roger |
| Author | Romano, Jonathan |
| Author | Kofman, Camila |
| Author | DeFoe, Alysse |
| Author | Kim, Yejun |
| Author | Anderson-Lee, Jeff |
| Author | Fisker, Eli |
| Author | Townley, Jill |
| Author | D'Aquino, Anne |
| Author | Das, Rhiju |
| Author | Jewett, Michael |
Subjects
| Subject | Synthetic biology |
|---|---|
| Subject | RNA design |
| Subject | citizen science |
| Genre | Data |
| Genre | Database |
| Genre | Image |
| Genre | Data sets |
| Genre | Dataset |
| Genre | Databases |
Bibliographic information
Access conditions
- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution 4.0 International license (CC BY).
Preferred citation
- Preferred citation
- Kruger, A., Watkins, A., Wellington-Oguri, R., Romano, J., Kofman, C., DeFoe, A., Kim, Y., Anderson-Lee, J., Fisker, E., Townley, J., D'Aquino, A., Das, R., and Jewett, M. (2022). Supplementary data on Eterna-designed superfolder ribosomes. Stanford Digital Repository. Available at https://purl.stanford.edu/hp267yt1382
Collection
Stanford Research Data
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- Contact
- andy.watkins2@gmail.com
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