The Junctional Epithelium Organoid: A Novel System for Periodontitis Research
Abstract/Contents
- Abstract
- Periodontitis is an ultra-prevalent oral disease affecting more than half of the US population. The disease is characterized by the breakdown of the Junctional Epithelium (JE), an oral tissue with important barrier and antimicrobial functions in the mouth. The vast majority of research on JE dysfunction is conducted using animal models. However, these animal models have deficiencies replicating all the aspects of the disease. In this work we describe the process of differentiating murine stem cells toward a JE organoid aimed to replicate the regional identity of the JE particularly the proliferative and anti-microbial functions of the organ. This project draws on the recent finding that the JE is maintained by its own stem cell population. This project aimed to develop a JE organoid from stem cells harvested from a murine model. Within the last 5 years the number of researchers that commonly use the organoid culture model has increased rapidly. Organoids have been grown mimicking brain, kidney, lung, intestine, stomach, and liver tissue. Organoids derived from patient biopsies faithfully replicate critical aspects of human disease; consequently, organoids can be used for drug screening. Similar to other organoid models, a validated JE organoid model could also be used to carry out a deep, unbiased interrogation of other unknown signaling pathways that participate in JE homeostasis. The development of a validated JE organoid system would represent a significant advance in the study of periodontal diseases.
Description
| Type of resource | text |
|---|---|
| Publication date | May 4, 2023 |
Creators/Contributors
| Author | Dawid, Isaiah |
|---|---|
| Thesis advisor | Helms, Jill |
| Thesis advisor | Feldman, Jessica |
| Degree granting institution | Stanford University, Department of Biology |
Subjects
| Subject | Junctional Epithelium |
|---|---|
| Subject | Oral Epithelium |
| Subject | Stem Cells |
| Subject | Periodontitis |
| Subject | Organoid |
| Genre | Text |
| Genre | Thesis |
Bibliographic information
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- License
- This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International license (CC BY-NC).
Preferred citation
- Preferred citation
- Dawid, I. and Helms, J. (2023). The Junctional Epithelium Organoid: A Novel System for Periodontitis Research. Stanford Digital Repository. Available at https://purl.stanford.edu/hj275gh4950. https://doi.org/10.25740/hj275gh4950.
Collection
Undergraduate Theses, Department of Biology, 2022-2023
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- Contact
- isaiahd@stanford.edu
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