Studies of actomyosin-ring-independent cytokinesis in budding yeast
- In the budding yeast Saccharomyces cerevisiae, cytokinesis is achieved through the coordinated action of constriction of the contractile actomyosin ring (CAR), ingression of the plasma membrane, and formation of the septal cell wall. Formation of the CAR depends on Myo1, the sole type II myosin, but myo1∆ cells lacking the CAR are viable, indicating that the CAR is not essential for cytokinesis. Although most myo1∆ cells form disoriented and/or disorganized cleavage furrows and primary septa (PS), in some cells these structures are nearly normal. Among the proteins that are involved in the assembly, function, and/or coordination of the CAR and PS-formation machinery are those in the complex containing Cyk3, Hof1, and Inn1. To further investigate the mechanisms underlying the CAR-independent processes of cytokinesis, I focused on elucidating the roles of this protein complex and of the interactions among Cyk3, Hof1, Inn1, and other interacting proteins. First, I found (Chapter 2) that a direct Cyk3-Hof1 interaction plays an important role in regulating the function of Cyk3 (and probably also that of Hof1) in PS formation during cytokinesis. Second, I found (Chapter 3) that four additional Cyk3-interacting proteins are involved in the function of the Cyk3-Hof1-Inn1 complex and the formation of normal septa during cytokinesis. The work presented here furthers our understanding of the CAR-independent aspects of yeast cytokinesis, and it may shed light on similar pathways in other cell types that form cleavage furrows in the absence of a CAR.
|Type of resource
|electronic; electronic resource; remote
|1 online resource.
|Stanford University, Department of Genetics.
|Statement of responsibility
|Submitted to the Department of Genetics.
|Thesis (Ph.D.)--Stanford University, 2017.
- © 2017 by Meng Wang
- This work is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported license (CC BY-NC).
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