Figure data for PNAS publication: A localized adaptor protein performs distinct functions at the Caulobacter cell poles
Abstract/Contents
- Abstract
- Asymmetric cell division generates two daughter cells with distinct characteristics and fates. Positioning different regulatory and signaling proteins at the opposing ends of the predivisional cell produces molecularly distinct daughter cells. Here, we report a strategy deployed by the asymmetrically dividing bacterium Caulobacter crescentus where a regulatory protein is programmed to perform distinct functions at the opposing cell poles. We find that the CtrA proteolysis adaptor protein PopA assumes distinct oligomeric states at the two cell poles through asymmetrically distributed c-di-GMP: dimeric at the stalked pole and monomeric at the swarmer pole. Different polar organizing proteins at each cell pole recruit PopA where it interacts with and mediates the function of two molecular machines: the ClpXP degradation machinery at the stalked pole and the flagellar basal body at the swarmer pole. We discovered a binding partner of PopA at the swarmer cell pole that together with PopA regulates the length of the flagella filament. Our work demonstrates how a second messenger provides spatio- temporal cues to change the physical behavior of an effector protein, thereby facilitating asymmetry.
Description
| Type of resource | software, multimedia |
|---|---|
| Date created | [ca. 2015 - 2020] |
Creators/Contributors
| Author | Wang, Jiarui | |
|---|---|---|
| Principal investigator | Shapiro, Lucy | |
| Principal investigator | W. E. Moerner |
Subjects
| Subject | Department of Chemistry Department of Developmental Biology (School of Medicine) |
|---|---|
| Genre | Dataset |
Bibliographic information
| Related Publication | https://doi.org/10.1073/pnas.2024705118 |
|---|---|
| Location | https://purl.stanford.edu/gx629ky7112 |
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- Use and reproduction
- User agrees that, where applicable, content will not be used to identify or to otherwise infringe the privacy or confidentiality rights of individuals. Content distributed via the Stanford Digital Repository may be subject to additional license and use restrictions applied by the depositor.
- License
- This work is licensed under a Creative Commons Attribution Non Commercial No Derivatives 3.0 Unported license (CC BY-NC-ND).
Collection
Stanford Research Data
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- Contact
- wmoerner@stanford.edu
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